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. 2021 Jan 28;9:598364. doi: 10.3389/fcell.2021.598364

FIGURE 2.

FIGURE 2

The changes of SIRT1 in decidualization of human ESC. (A) Immunofluorescence staining of SIRT1 (green) and vimentin (red) were performed human ESCs. The nuclear were stained with DAPI (4′,6-diamidino-2-phenylindole) (blue). Scale bar is 50 μm. (B) The mRNA levels of PRL and IGFBP1 during decidualization (0, 2, and 4 days) in vitro. mRNA expression levels were normalized to β-actin. Bar graph was the average data of three independent experiment. (C) A cytoskeletal staining of α-tubulin (red) demonstrated differences in cell shape and cytoskeletal architecture. The nuclear were stained with DAPI (4′,6-diamidino-2-phenylindole) (blue). Scale bar is 50 μm. (D) The mRNA levels of IGFBP1 and PRL in ESC during decidualization in vitro from CTRL (n = 7) and RIF (n = 7). (E) Western blotting analysis of SIRT1 protein abundance in ESC during decidualization in vitro from CTRL (n = 7) and RIF (n = 7), blots graph was representative and bar graph was the average data. *P < 0.05, **P < 0.01, and ****P < 0.0001 vs. 0 days or CTRL (data are means ± SEM).