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. 2021 Jan 28;9:598364. doi: 10.3389/fcell.2021.598364

FIGURE 5.

FIGURE 5

The effect of SIRT1 deficiency on ROS homeostasis through FOXO1 in ESCs. (A) Western blotting analysis of FOXO1 protein levels between siNC and siSIRT1 groups. Blots graph was representative and bar graph was the average data of three independent experiments. Scale bar is 50 μm. (B) Changes in intracellular ROS abundance between siNC and siFOXO1 groups with or without SRT1720 (10 μM) treatment. Bar graph was the average data of three independent experiments. (C) Western blotting analysis of FOXO1, SOD2 and NRF2 protein in siNC, siNC+SRT1720, siFOXO1, siFOXO1 + SRT1720 groups. Blots graph was representative and bar graph was the average data of three independent experiments. (D) The mRNA levels of decidual biomarkers in above four groups. mRNA expression levels were normalized to β-actin. Bar graph was the average data of five independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 (data are means ± SEM).