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. 2021 Jan 28;9:598364. doi: 10.3389/fcell.2021.598364

FIGURE 6.

FIGURE 6

The role of SIRT1 on FOXO1 translocation by deacetylation in decidual ESCs. (A) Co-IP assay was used to examine the association between SIRT1 and FOXO1. Input and IgG served as positive and negative controls, respectively. (B) Western blotting analysis of SIRT1, Acetyl-FOXO1 abundance after 4-day induced decidualization in siNC, siSIRT1, siSIRT1 + SRT1720 groups. Blots graph was representative and bar graph was the average data of three independent experiments. (C) Microscopy images showing immunofluorescence localization of FOXO1 (green) in above three groups; the nuclear were stained with DAPI (blue). Scale bar is 50 μm. (D) The representative blot of SIRT1, Acetyl-FOXO1, and FOXO1 proteins in nuclear and cytoplasm of decidual ESCs in above three groups. Blots graph was representative and bar graph (E) was the average data of three independent experiments. (F) Quantification of the Western blotting assays of FOXO1 distribution and FOXO1 translocation from nuclear to cytoplasm. The nucleoprotein and cytoplasmic protein levels were normalized to Lamin A/C and β-actin, respectively. Bar graph was the average data of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 (data are means ± SEM).