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. 2021 Feb 10;7:31. doi: 10.1038/s41420-021-00404-4

Fig. 2. Inhibition of IP3R1 attenuates H/R-induced pyroptosis and inflammation in cardiomyocytes.

Fig. 2

A RT-qPCR was used to detect the efficiency of siRNA transfection; B CCK-8 was used to detect cell viability; C Western blot was used to detect the levels of pyroptosis-related proteins; D colorimetric method was used to detect the activity of Caspase-1; E ELISA was used to detect the levels of IL-1β and IL-18. Cardiomyocytes are isolated and cultured from adult rat myocardium. Compared to the blank group, *p < 0.05, **p < 0.01, ***p < 0.001; for pairwise comparison, #p < 0.05, ##p < 0.01, ###p < 0.001. Data in panels A/D were analyzed using one-way ANOVA and data in panels B/C/E were analyzed using two-way ANOVA. Tukey’s multiple comparison test was applied for the post hoc test after ANOVA.