Fig. 4. Binding of ERP44 to IP3R1 inhibits the I/R-induced mitochondrial Ca²⁺ overload.

A, C Western blot was used to detect the level of ERP44 protein; B immunoprecipitation was used to detect the binding relationship between IP3R1 and ERP44; D Rhod2 AM fluorescent labeling was used to observe the changes of intracellular Ca²⁺ level. Compared to the blank group, *p < 0.05, ***p < 0.001; for pairwise comparison, ^##p < 0.01, ^###p < 0.001. All experiments were performed three times independently. Data were analyzed using one-way ANOVA and Tukey’s multiple comparison test.