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. 2021 Feb 10;7:31. doi: 10.1038/s41420-021-00404-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A CCK-8 was used to detect cell viability; B colorimetric assay was used to detect Caspase-1 activity; C western blot was used to detect the levels of pyroptosis proteins; D ELISA was used to detect the levels of IL-1β and IL-18. Nif represents calcium channel blocker Nifedipine D6. Compared to the H/R group, **p < 0.01, ***p < 0.001. All experiments were performed three times independently. Data in panel B were analyzed using one-way ANOVA and data in panels A/C/D were analyzed using two-way ANOVA. Tukey’s multiple comparison test was applied for the post hoc test after ANOVA.