Figure 12.

Human TM cells grown for 48 hours on hydrogels of various stiffnesses (2-75 kPa). Conditioned serum-free media was subject to SDS-PAGE and Western immunoblots and probed with a versican monoclonal antibody (12C5; Developmental Studies hybridoma Bank, University of Iowa, Iowa). Densitometry was used to quantitate each of the gel bands and the relative fluorescent units (RFUs) were averaged. The line in the plots shows the median with N=6 technical replicates from 4 normal TM cell strains. TCP = tissue culture plastic; TCP + FNC = tissue culture plastic coated with fibronectin-collagen mixture that was used to coat the hydrogels. Isoforms V1 and V2 are shown.