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. 2021 Jan 28;11:618992. doi: 10.3389/fphar.2020.618992

FIGURE 5.

FIGURE 5

LPS-MCM triggered the aberrant expression of kir6.2 in primary astrocytes. (A) Protocol of treatment for (B–E). Primary microglia were stimulated with 100 ng/ml LPS for 24 h to collect the MCM. Primary astrocytes were cultured with the MCM diluted at a ratio of 1:3 for 24 h to conduct bioassay. (B) Protein levels of kir6.2 and C3 were detected by western blotting. (C) Densitometric analysis of kir6.2 and C3. Data are analyzed by unpaired Student’s test. **p < 0.01 and ***p < 0.001 vs. CON group. (D) Immunofluorescent stainings of GFAP (green) and kir6.2 (red) in primary astrocytes.