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. 2021 Jan 28;9:623175. doi: 10.3389/fcell.2021.623175

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Copyright © 2021 de Mera-Rodríguez, Álvarez-Hernán, Gañán, Martín-Partido, Rodríguez-León and Francisco-Morcillo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SA-β-GAL activity and cell death in the embryonic day E9 (A) and E10 (B) chicken retina. Cryosections were doubly stained with SA-β-GAL histochemistry and TUNEL technique. TUNEL-positive nuclei are mainly detected in the GCL (arrowheads) and in the middle region of the INL (arrows). SA-β-GAL activity is observed in the GCL, amacrine cell layer, and horizontal cell layer. ac, amacrine cells; gc, ganglion cells; GCL, ganglion cell layer; hc, horizontal cells; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; pos, photoreceptor outer segments. Scale bar: 50 μm.