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. 2021 Jan 28;11:622389. doi: 10.3389/fmicb.2020.622389

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Copyright © 2021 Rao, Su, Peng, Guo, Huang, Ye, Gao, Liu, Zhang, Luo and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The numbers of interferon-γ-secreting T cells (ISCs) were increased after the removal of low-density granulocytes (LDGs). (A) The LDGs in peripheral blood mononuclear cells (PBMCs) of tuberculosis patients (n = 16) were removed by immunomagnetic separation, and T-SPOT assays were performed with the untreated PBMCs and LDG-removed PBMCs, respectively, with the same numbers of mononuclear cells. The ISCs in response to (B) ESAT-6 and (C) CFP-10 stimulation, and (D) the sum of the ISCs in response to ESAT-6 and CFP-10 were counted and compared by paired Student’s t-test. (E) The positive rates of T-SPOT assay were compared by chi-square test.