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. 2021 Jan 28;11:622389. doi: 10.3389/fmicb.2020.622389

FIGURE 7.

FIGURE 7

Blocking programmed death ligand 1 (PD-L1) signal increased the number of interferon-γ-secreting T cells (ISCs). Peripheral blood mononuclear cells (PBMCs) were collected from tuberculosis patients (n = 11) and treated with anti-PD-L1 monoclonal antibody or removal of low-density granulocytes (LDGs) by immunomagnetic bead method. These pretreated PBMCs were used together with untreated PBMCs for T-SPOT assay. The number of ISCs in response to (A) ESAT-6 and (B) CFP-10 and (C) the total number of ISCs in response to both ESAT-6 and CFP-10 were counted. LDGs from tuberculosis patients (n = 12) were isolated and treated with or without anti-PD-L1 monoclonal antibody, followed by adding into autologous freshly collected PBMCs. These PBMCs with exogenous LDG were used together with untreated PBMCs for T-SPOT assay. The number of ISCs in response to (D) ESAT-6 and (E) CFP-10 and (F) the total number of ISCs in response to both ESAT-6 and CFP-10 were counted. Statistical significance was determined by paired Student’s t-test.