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. 2020 Dec 1;3(1):fcaa211. doi: 10.1093/braincomms/fcaa211

Figure 1.

Figure 1

Study protocol. Baseline vital signs were taken prior to induction of anaesthesia and surgical preparation. Following surgery, all piglets underwent cerebral HI by inflation of carotid occluders and reduction of inspired oxygen to 6%. Piglets were resuscitated after HI and observed for 1 h prior to randomization to (i) HT + vehicle (HT+V), (ii) HT+MEL (MEL) double therapy, (iii) HT + Epo double therapy or (iv) HT+MEL+Epo triple therapy. All piglets were cooled to 33.5°C for 12 h from 1 h after HI. MEL 20 mg/kg was infused intravenously over 2 h and Epo 3000 units/kg was given as an intravenous bolus. These were given at 1 h, 24 h and 48 h after HI. The HT+V group received vehicle infusion at an equivalent volume and rate as MEL and a bolus of 0.9% sodium chloride at the same volume as Epo. MRS was acquired out-of-hours at 30 h (18:00) and 66 h (06:00) using the clinical 3 T Philips Achieva magnetic resonance scanner. Cerebral electrical activity was continuously monitored with aEEG. Studies were terminated at 72 h.