Figure 2.

CD5 levels correlate with PD-1, CD69, and CTLA-4 in lymphoid organs. Splenocytes and lymphocytes isolated from 4T1-harboring BALB/c mice were stained with fluorescence-conjugated antibodies to assess PD-1, CD69, and CTLA-4 in correlation to CD5. (A) The fraction of PD-1⁺/CD5^high/CD4⁺, CD69⁺/CD5^high/CD4⁺, CTLA-4⁺/CD5^high/CD4⁺ T, PD-1⁺/CD5^low/CD4⁺, CD69⁺/CD5^low/CD4⁺, and CTLA-4⁺/CD5^low/CD4⁺ T cells in spleen and lymph nodes, respectively. (B) The fraction of PD-1⁺/CD5^high/CD8⁺, CD69⁺/CD5^high/CD8⁺, CTLA-4⁺/CD5^high/CD8⁺ vs PD-1⁺/CD5^low/CD8⁺, CD69⁺/CD5^low/CD8⁺, and CTLA-4⁺/CD5^low/CD8⁺ T cells in spleen and lymph nodes, respectively. Data are shown as means ± SD of 5 or 3 mice per group and from one representative experiment of three independent experiments. *p < 0.05 (Student’s paired two-tailed t-test. ns, not significant).