Figure 3.

CD5 levels and their correlation with PD-1, CD69 and CTLA-4 in TILs. TILs isolated from 4T1-harboring BALB/c mice were stained with fluorescence-conjugated antibodies to assess PD-1, CD69 and CTLA-4 in correlation with CD5. (A) The fraction of PD-1⁺/CD5^high/CD4⁺, CD69⁺/CD5^high/CD4⁺, CTLA-4⁺/CD5^high/CD4⁺ vs PD-1⁺/CD5^low/CD4⁺, CD69⁺/CD5^low/CD4⁺, and CTLA-4⁺/CD5^low/CD4⁺ TILs. (B) The fraction of PD-1⁺/CD5^high/CD8⁺, CD69⁺/CD5^high/CD8⁺, CTLA-4⁺/CD5^high/CD8⁺ vsPD-1⁺/CD5^low/CD8⁺, CD69⁺/CD5^low/CD8⁺, and CTLA-4⁺/CD5^low/CD8⁺ TILs. Data are shown as means ± SD of 4 or 3 mice per group and from one representative experiment of three independent experiments. *p < 0.05 (Student’s paired two-tailed t-test. ns, not significant).