Figure 4.

Sleep-wake behavior of single fly. Measures of daily total activity, daily total sleep and night activity offset in the DM obtained with Appl-Gal4 driver (A) and D42-Gal4 driver (B) and corresponding Controls have been done in male flies at different ages as indicated in Supplementary Table 3. Data are scaled to both chronological age and physiological age. All measurements were performed at Zeitgeber time 0 (ZT0), the time when the lights go “on.” Results are represented for each single measurement of two experiments with independent cohorts with mean values. The abbreviations and genotype of flies are described in Table 1. To evaluate the difference in age-dependent changes in sleep-wake behavior parameters between Control and DM lines, regression curve slopes and intercepts were compared (Table 4).