FIG. 6.

Activation of mitochondrial spare capacity by osteoinduction requires active AKT. (A) Energy map of ST2 (top) and MC3T3-E1 (bottom) showing baseline (B: black outlined) and maximal (U: red outlined) OCR and ECAR of cells incubated with Akt inhibitor (iAkt) for 24 h and then induced using OBM (left), BMP2 (center), or Wnt3a (right) addition. Data represented as fold change over uninduced control cells. (B, C) ALP stain for cells treated with or without iAkt for 24 h before OB induction with OBM, BMP2, or Wnt3a for 4 days. Seahorse n = 15. Data are mean ± SD (n = 15). Statistical significance of conditions versus uninduced control was determined by ANOVA and confirmed by post hoc Bonferroni correction. No significance was found. Color images are available online.