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. 2021 Jan 28;10:596023. doi: 10.3389/fcimb.2020.596023

Figure 2.

Figure 2

Group A Streptococcus (GAS) infection induced neutrophil death. Human neutrophils (PMNs) were stained with Annexin V-FITC and Zombie Aqua Fixable Viability dye via flow cytometry and initially gated upon (A) ‘singlets’ (FSC-A vs. FSC-H, 10,000 events collected). ‘All PMNs’ (live and dead) were selected using (B) a gate excluding GAS. Cells were then compared for fluorescence of (C) annexin V-FITC/phosphatidylserine (PS) binding against Zombie live/dead viability dye. Purified PMNs were sampled over 180 min to measure (D), ‘viable’ (AV-Z-), (E) ‘PS exposed’ (AV+Z-) and (F) ‘dead’ (AV+Z+), neutrophils during GAS infection (n=3 donors). Results are pooled means ± SD. Linear mixed model ‘p’ values represent interaction (treatment*time) or are stated. *p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001, with black asterisks denoting significance from control and grey asterisks between 5448 and 5448AP.