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. 2021 Jan 28;10:596023. doi: 10.3389/fcimb.2020.596023

Figure 5.

Figure 5

Group A Streptococcus (GAS) infection in vivo activates caspases in murine neutrophils. Percentage of CD45+ cells (A) lavaged from site of injection and in (B) circulating blood characterised as neutrophils (PMNs, CD11b+/Ly-6G+) by flow cytometry ( Figures S4A–E ). (C) Inflammatory caspase activation in neutrophils at site of GAS infection was confirmed via flow cytometry using FLICA 660 (660-YVAD-FMK, Figure S4F ). Each data point represents the results from a single mouse where n=8. (D) The release of IL-1β in mouse serum during GAS infection was measured using the LEGENDplex™ mouse inflammation cytometric bead assay at 6 and 24 h post-infection where mean of duplicate measurements are shown and n=8. (E) CD16 expression of PMNs lavaged from the site of injection and determined using flow cytometry. (F) Bacteria were recovered from the site of infection and concentration determined by plating and colony enumeration where post-hoc analysis was performed using the Holm-Šídák approach. Each data point represents the results from a single mouse where n=8. Results are the means ± SD. Two-way ANOVA ‘p’ values represent interaction (treatment*time) or are stated.*p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001, with black asterisks denoting significance from control and grey asterisks between 5448 and 5448AP, or as indicated by a line.