Table 1.
Theoretical pI, protein yield, melting temperature (Tm), and percent refolding of the CC3-hop variants.
| Clone | Theoretical pI | Yield mg/L | Tm (°C) | % refold |
|---|---|---|---|---|
| CC3-hop | 7.75 | 12.4 | 62 | 80 |
| CC3-m1hop | 6.12 | 28 | 76 | 95 |
| CC3-m2hop | 6.35 | 18 | 69 | 91 |
| CC3-m3hop | 6.63 | 9.9 | 72 | 91 |
| CC3-m4hop | 7.04 | 9.2 | 70 | 81 |
| CC3-m5hop | 8.40 | 20.5 | 58 | 46 |
| CC3-m6hop | 8.75 | 9.6 | 55 | 38 |
| CC3-m7hop | 9.0 | 11 | 55 | 19 |
| CC3-m8hop | 9.18 | 11 | 56 | 19 |
| CC3-m9hop | 9.35 | 5.8 | 54 | 5 |
| CC3-m10hop | 9.51 | 3.4 | 49 | 15 |
The Tm and percent refolding for the mutants with a more negative pI than CC3-hop are shown in bold. A representative protein yield is show; although variation is seen between preparations, typically very similar yields are observed. The error on Tm is ± 1°C.