Fig. 4.

Site-specific N-glycosylation profile of RBDs expressed in human and insect cells. A, the glycosites and deduced representative N-glycans on N331 and N343 of RBD. B and C, different types and numbers of N-glycans on N331 and N343 of RBD expressed in insect cells (B) or human cells (C). The size of the pies represents the number of N-glycan compositions. D, comparison of N-glycans on N331 and N343 between RBD and S ectodomain expressed in insect cells. E, comparison of N-glycans on N331 and N343 between RBD and S1 subunit expressed in human cells.