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. 2021 Jan 13;11(2):386–394. doi: 10.1002/2211-5463.13049

Fig. 2.

Fig. 2

The promoter activity of RNF10 is regulated by TRIF. (A) RNF10 promoter activity in L929 cells treated with LPS for 0.5 and 1 h. (B) RNF10 promoter activity in L929 cells transfected with si‐ctrl, si‐TRIF or si‐Myd88 for 48 h before LPS stimulation for 1 h. (C) Quantitative‐PCR analysis of RNF10 expression in wild‐type, Ticaml–/– and Myd88−/− RAW264.7 cells. (D) Immunoblot analysis of TRIF in peritoneal macrophages from young and aged mice stimulated with LPS for the indicated time. Data are representative of independent experiments. Data are shown as the mean ± SD (n = 3). *P < 0.05, **P < 0.01 (Student’s t‐test).