Fig. 4.

Molecular mechanism of PLCγ1 inhibition combined with inhibition of apoptosis and necroptosis increases cartilage matrix synthesis in IL‐1β‐treated rat chondrocytes. Inhibition of PLCγ1 phosphorylation by U73122 improved cartilage matrix synthesis in IL‐1β‐treated rat chondrocytes. However, it also increased cell apoptosis and necroptosis, which reduced the effect on cartilage matrix synthesis. Inhibition of programmed cell death by simultaneous treatment with the apoptosis and the necroptosis inhibitor increased chondrocyte proliferation and further enhanced cartilage matrix synthesis.