Fig. 1.

lncRNA DLGAP1‐AS2 was up‐regulated in CCA and contributes to promoting cell viability of CCA cells. (A) Bioinformatics analysis of DLGAP1‐AS2 expression in CCA tissues and normal tissues. P < 0.0001. (B) The levels of DLGAP1‐AS2 in CCA cell lines HUCCT1 and RBE, as well as normal HEBEpics cells. (C) Kaplan–Meier survival analysis of the relevance of DLGAP1‐AS2 expression and survival rate of patients with CCA. P = 0.0019. (D,E) The transfection efficiency of si‐DLGAP1‐AS2#1 and si‐DLGAP1‐AS2#2 in HUCCT1 and RBE cells was measured using qRT‐PCR. (F,G) The impacts of si‐DLGAP1‐AS2#1 and si‐DLGAP1‐AS2#2 on cell viability of HUCCT1 and RBE cells were detected using CCK‐8 assay. **P < 0.01. Error bar represents the mean ± SD derived from three independent experiments. Comparisons between groups were analyzed using t‐tests (two‐sided). OD, absorbance.