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. 2021 Feb 9;218(4):e20201189. doi: 10.1084/jem.20201189

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© 2021 Reitermaier et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S2. — Comparative analysis of T cells in fetal organs and PBMCs. (A and B) Representative images of cell types showing brightfield (BF) and immunofluorescence (CD3/αβ/γδ/Vδ1/Vδ2/CD4/CD8) as analyzed using ImageStream (n = 4). (C and E) Representative biaxial plots demonstrating the gating of T cell subsets in fetal organs and PBMCs as well as analysis by ImageStream and flow cytometry (n = 5). (D) Representative confocal laser microscopy images showing a T cell marker expression profile in indicated fetal organs (n = 4). Scale bar, 10 µm.