Figure 6.

Intravenous lidocaine inhibits the activation of astrocyte but not microglia in CA1

(A) Immunofluorescence images of microglia in CA1 (bregma −1.58 mm).

(B and C) (B) Proportion of microglia-fluorescent area and (C) number of microglia in CA1, which indicated that the activated microglia by RTX cannot be inhibited by lidocaine.

(D) The microglia soma size comparisons in CA1, which indicated that the activated microglia cannot be reversed by lidocaine.

(E) Immunofluorescence images of astrocytes in CA1 (bregma −1.58 mm).

(F) Proportion of astrocyte-fluorescent area in CA1, which indicated that the activated astrocytes can be reversed by lidocaine. The proportion, number of microglia and astrocytes, and the cell soma sizes were calculated with ImageJ. Data were expressed as mean ± SEM, n = 5–6, 5 slices for each rat. Statistical analyses consisted of one-way ANOVA tests followed by Tukey's post-hoc tests. Scale bars, 50 μm. ^#p < 0.05, ^##p < 0.01, ∗p < 0.05, ∗∗p < 0.01, n.s = not significant.