REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-PD1	Abcam	Cat# ab214421
Mouse monoclonal anti-TRAIL	Abcam	Cat# ab10516
Goat anti-rabbit IgG (H+L), HRP conjugate	TransGen Biotech	Cat# HS101-01
Goat anti-mouse IgG (H+L), HRP conjugate	TransGen Biotech	Cat# HS201-01
Bacterial and virus strains
Trans1-T1 phage resistant chemically competent cell	TransGen Biotech	Cat# CD501-02
Chemicals, peptides, and recombinant proteins
DMEM	Gibco	Cat# 11995500
Opti-MEM	Gibco	Cat# 31985-LSG
FBS	PAN	Cat# P30-3302
PBS	HyClone	Cat# SH30256.01
Penicillin-streptomycin	Gibco	Cat# 15140122
Ampicillin	Solarbio	Cat# YZ-1033000 Cas# 69-53-4
Kanamycin	Solarbio	Cat# YZ-1355006 Cas# 5965-95-7
Tryptone	OXOID	Cat# LP0042
Yeast extract	OXOID	Cat# LP0021
NaCl	Sinopharm	Cat# 73522260 Cas# 7647-14-5
DiO	Beyotime	Cat# C1038
DiI	Beyotime	Cat# C1036
DAPI	Sigma-Aldrich	Cat# D9542 Cas# 28718-90-3
PMSF	Beyotime	Cat# ST506
Protease inhibitor cocktail	Sigma-Aldrich	Cat# P8340
Polybrene	Santa Cruz	Cat# SC-134220
Paraformaldehyde, 4%	Solarbio	Cat# P1110
RIPA lysis buffer	Servicebio	Cat#G2002
Critical commercial assays
EndoFree Maxi Plasmid Kit	TIANGEN BIOTECH (BEIJING)	Cat# DP117
Easy II Protein Quantitative Kit	TransGen	Cat# DQ111-01
Membrane and Cytosol Protein Extraction Kit	Beyotime	Cat# P0033
Lipofectamine 3000 Transfection Kit	Thermo Fisher	Cat# L3000015
Mouse PD-1 Duo Set ELISA Kit	R&D Systems	Cat# DY1021
Mouse TRAIL/TNFSF10 Duo Set ELISA Kit	R&D Systems	Cat# DY1121
Experimental models: cell lines
Human: HEK293FT	ATCC	N/A
Recombinant DNA
Plasmid: pCDH-CMV-MCS-EF1-Puro	This paper	N/A
Plasmid: pCDH-CMV-MCS-EF1-Blasticidin	This paper	N/A
Plasmid: pCDH-CMV-PD1-mCherry-Puro	This paper	N/A
Plasmid: pCDH-CMV- TRAIL-EGFP-Blasticidin	This paper	N/A
Software and algorithms
Flowjo	Tree Star	10.0
Zen	Carl Zeiss	2012 SP2(blue edition)
Other
Confocal dish	NEST	Cat# 801005
6-well plate	Costar	Cat# 3335
0.22 μm filter	Merck Millipore	Cat#PRO3683
15 mL centrifuge tubes	Corning	Cat# 430790
25 cm2 cell culture flasks	Corning	Cat# 430639
75 cm2 cell culture flasks	Corning	Cat# 430641
Cell scraper	Thermo Fisher	Cat# 179693PK
CO2 incubator	Thermo Scientific	HERAcell150i
Thermostat water bath	Jinghong	DK-8D
Clean bench	AIRTECH	SW-CJ-2FD
Autoclave sterilizer	ZEALWAY	GR85DR
Milli-Q gradient system	Milli-Q Reference	Milli-Q Integral 3
NanoDrop2000/2000c spectrophotometer	Thermo Scientific	ND2000LAPTOP
Ultracentrifuge	Beckman Coulter	Optima XPN-100
Centrifuge	Thermo Fisher	Sorvall ST 16R
Dry bath	ZHISUN	Mk-20
Avanti mini-syringe	Avanti	610017-1Ea
Confocal laser scanning microscope	Zeiss	LSM780
Flow cytometry	BD	FACSVerse
Fluorescence microscope	Zeiss	Axio Vert.A1