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. 2020 Oct 23;104(2):479–491. doi: 10.1093/biolre/ioaa196

Figure 6.

Figure 6

Effects of functional inhibition of HIF1alpha or suppression of its expression, on oocyte maturation rates (IVM output) and steroidogenic output (progesterone, P4), and on blastocyst rates (IVP output) from bovine COCs treated with HIF1alpha blockers. COCs were treated with either echinomycin (5 nM) or PX-478 (25 and 50 μM) and the IVM output was determined compared with untreated COCs (IVM/control) (A and C). In parallel experiments, control COCs and those treated with blockers were further used for IVF, and IVP output was determined by comparing numbers of expanded and hatched blastocysts at day 8 of culture (A and C). The steroidogenic output was determined by RIA in media from control and echinomycin-treated COCs (B). Student t-test, unpaired, two-tailed, was applied in (A) and (B), and one-way ANOVA was applied in (C), separately for IVM and IVP output (mean ± SEM). Following ANOVA (P < 0.002 and 0.001 for IVM/IVP output, respectively), Tukey–Kramer multiple comparisons post-test was applied to test the effect of the treatment on gene expression (solid lines). Bars with asterisks differ with: *P < 0.05, **P < 0.01, and ***P < 0.001.