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. 2020 Oct 13;104(2):445–456. doi: 10.1093/biolre/ioaa192

Figure 5.

Figure 5

Oxidative stress inhibits local P4 release in AMCs. (A) Although all fetal membrane cells can produce progesterone, chorion cells produce higher levels compared with amnion epithelial and mesenchymal cells (AEC: 0.6 ± 0.1 ng/mL, AMC: 2.5 ± 1.2 ng/mL, chorion: 58.8 ± 8.1 ng/mL). In addition, full fetal membrane explants secrete significant levels of P4, which is likely produced by the chorion layer (P < 0.0001) (chorion: 58.8 ± 8.1 ng/mL, decidua: 2.3 ± 0.1 ng/mL) (N = 3; mean ± SEM). To compare cell types under normal cell culture conditions, we extrapolated and normalized P4 production for 6 days within a T75 flask. (B and D) CSE-induced oxidative stress did not affect P4 production in AECs (B), CSE decreased P4 production in AMCs (C) (P = 0.039; control: 59.2 ± 8.43 pg/mL, CSE: 28.95 ± 12.5 pg/mL) and chorion cells (D) (control: 1192 ± 401.6 pg/mL, CSE: 953.9 ± 350 pg/mL) (N = 3; mean ± SEM).