Fig. 3.

Stable silencing of ESCO2 expression inhibits the malignant phenotype of LUAD cells in vitro, and growth and metastasis in vivo. a and b ESCO2 expression was stably silenced using shRNA; ESCO2 mRNA and protein expression levels in NCI-H1975 and A549 cells were detected by q-PCR (a) and western blotting (b), respectively. c–e The effects of ESCO2 stable silencing on LUAD cell growth (c), migration and invasion (d), and colony formation (e) were detected. f The in vivo growth of NCI-H1975 cell lines with ESCO2 stable silencing was detected (n = 6). g NOD-SCID mice (n = 5) were transplanted with Luc-labeled NCI-H1975 cells (2 × 10⁶ cells/mouse) with ESCO2 stable silencing to detect in vivo metastasis ability. h The lung metastatic nodule number was analyzed. Data are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001