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. 2021 Feb 10;21:100. doi: 10.1186/s12935-021-01803-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — a CD133⁺ CSCs were enriched from human HT-29 cell line using MACS and the purity of isolated cells assessed by FITC-conjugated anti-CD133 antibody and flow cytometry analysis. b The chemical structure of T0901317. c Measuring CSCs survival after exposure to various concentrations of T0901317 (5–200 µM) using MTT assay. d The chemical structure of SR9243. e CSCs survival rate was also measured after treatment with SR9243 (25–200 nM) using MTT assay. Data confirmed a dose-dependent activity of T0901317 and SR9243 on HT-29-isolated CSCs