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. 2021 Feb 10;21:100. doi: 10.1186/s12935-021-01803-4

Table 1.

Primer list

Gene Forward primer Reverse primer Tm (°C)
ABCA1 TTCCCGCATTATCTGGAAAGC CAAGGTCCATTTCTTGGCTGT 57.9
ABCG8 AGCCTCCTTGCTAGATGTGAT GTCTCTCGCACAGTCAAGTTG 57.9
ABCG5 ACTGCTTCTCCTACGTCCTG CTGTAGTTGCCAATCAGTCGG 59.4
SCD1 TCTAGCTCCTATACCACCACCA TCGTCTCCAACTTATCTCCTCC 60.3
FASN AAGGACCTGTCTAGGTTTGATGC TGGCTTCATAGGTGACTTCCA 60.6
GSK3β CCGACTAACACCACTGGAAGCT AGGATGGTAGCCAGAGGTGGAT 59.8
PFKFB3 ATTGCGGTTTTCGATGCCAC GCCACAACTGTAGGGTCGT 58.8
HIF1A-F ATCCATGTGACCATGAGGAAATG TCGGCTAGTTAGGGTACACTTC 60.3
GAPDH GGAGCGAGATCCCTCCAAAAT GGCTGTTGTCATACTTCTCATGG 59.8