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. 2021 Feb 15;18(1):139–154. doi: 10.20892/j.issn.2095-3941.2020.0151

Figure 2.

Figure 2

Mass spectrometric analyses highlight the upregulation of mitochondrial associated protein expression by vascular endothelial growth factor receptor (VEGFR)2 blockade. MCF-7 breast cancer cells were cultured in the presence of vehicle (0.01% dimethyl sulfoxide), 2.5, and 5 μM Ki8751 for 24 h. Whole cell lysates were prepared with Laemmli buffer containing 10 mM dithiothreitol and 5% 2-mercaptoethanol. Quantitative proteomics analyses of proteome regulation were performed using nanoLC-MS/MS among the three differently treated MCF-7 cells. Panel A: Orthogonal projections to latent structures discriminant analysis of protein abundances to reveal the differences among differently treated MCF-7 cells. B: The heat map of the top 50 up- and downregulated proteins of MCF-7 cells treated with 5 μM Ki8751. C: Correlation of significantly regulated proteins in MCF-7 cells treated with 2.5 and 5 μM Ki8751. D and E: The relative abundances of the three most up- and downregulated proteins. *P < 0.05; **P < 0.01; ***P < 0.001, as compared to the control (vehicle-treated) cells; n = 3.