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. 2021 Feb 15;18(1):139–154. doi: 10.20892/j.issn.2095-3941.2020.0151

Figure 5.

Figure 5

Vascular endothelial growth factor receptor (VEGFR) 2 blockade by Ki8751 arrests mitosis of breast cancer cells. The breast cancer cells, MCF-7 and MDA-MB-231, were cultured in 12-well plates at 5 × 104 cells/well. After 24 h, vehicle, 2.5, or 5 μM Ki8751 were added, and the cells were further cultured for 24, 48, and 72 h. The cells were stained with propidium iodide/RNase staining buffer. DNA content of the MCF-7 cells (panel A) and MDA-MB-231 cells (B) were analyzed using a NovoCyte flow cytometer. Cell cycle analyses (C and D) were performed using the NovoExpress® program.