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. 2021 Feb 15;18(1):88–104. doi: 10.20892/j.issn.2095-3941.2020.0096

Figure 2.

Figure 2

The efficiency of BYSL downregulation and overexpression in glioma cells. (A) The qRT-PCR revealed that different concentrations of BYSL siRNAs significantly downregulated the BYSL levels in U251 and U87 glioma cells. (B) Western blot analysis showed that 100 nM siRNA significantly decreased the BYSL protein levels in U251 and U87 glioma cells. (C) By combining the green fluorescent protein and 4,6-diamidino- 2-phenylindole staining, 90% of the cells were confirmed to be infected by lentiviruses produced from the vector and BYSL plasmids. Scale bar: 200 μm. (D) Western blot analysis showed that BYSL was abundantly expressed in U251 and U87 cells. M, molecular marker (*P < 0.05; **P < 0.01; ***P < 0.001).