Figure 6.

Morphine-3-glucuronide (M3G) undermines the function of CTL. (A) M3G significantly reduces the cytotoxicity of CTL in the CTL target-killing experiments towards the A549 cells at the ratios of 40:1 and 20:1 (CTL/A549) and this effect is reversed by TAK-242 treatment, n = 3 independent experiments. (B) The ELISpot experiment shows that A549 cells pretreated with 10 μM M3G for 24 h decreases the release of INF-γ from the cytotoxic T lymphocytes (CTLs) in the co-culture of CTLs and A549 at a ratio of 20:1. Left panel: representative images for ELISpot; right panel: quantification for the left panel, n = 3 independent experiments. Data are presented as the mean ± SEM. *P < 0.05, one-way analysis of variance with Bonferroni’s post hoc test.