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. 2021 Jan 28;12:633354. doi: 10.3389/fimmu.2021.633354

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Copyright © 2021 Zhang, Xie, Su, Liu, Zhang, Pang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of ISE on STAT3 signal. RAW264.7 macrophages were pretreated with different concentrations of ISE for 1h and then stimulated by 1.0μg/mL LPS or 3T3-L1CM for 30min. Total cell lysates were extracted, and then western blotting using specific antibodies was used to determine the expression of p-STAT3 and STAT3 in LPS (A) and 3T3-L1CM (L1CM) stimulation (B), respectively. The value of a control was set at 100%, and the relative value was presented as fold induction to that of the control, which was normalized to total STAT3. Statistical comparisons were made with each vehicle controls. The values are means ± SD, n = 3. *P < 0.05, **P < 0.01.