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. 2021 Jan 15;8(1):ENEURO.0286-20.2020. doi: 10.1523/ENEURO.0286-20.2020

Table 4.

Statistics table

Mean Effect (Cohen’s d) N
Figure Target Group comparison Data
structure		 Type of test Shuffles p value Difference 95% CI
lower		 95% CI
upper		 Effect 95% CI
lower		 95% CI
upper		 Power p < 0.05 UT TTX
a Gephyrin distance to synapsin1 Gephyrin positive synapses (UMAP 6 vs UMAP1-5) Normal Permutation t test 5000 0.002 −0.44 −0.46 −0.40 −16.60 −20.65 −12.49 1.0 * 6 5
3A,F Gephyrin integrated intensity Gephyrin positive synapses (UMAP 6 vs UMAP1-5) Normal Permutation t test 5000 0.002 0.35 0.20 0.49 2.70 1.25 5.46 1.0 * 6 5
PSD-95 distance to synapsin1 PSD-95 positive synapses (UMAP6 vs UMAP1-5) Normal Permutation t test 5000 0.003 −0.56 −0.70 −0.47 −5.67 −7.51 −4.11 1.0 * 6 5
3A,F PSD-95 integrated intensity PSD-95 positive synapses (UMAP6 vs UMAP1-5) Normal Permutation t test 5000 0.007 −0.23 −0.31 −0.10 −2.56 −6.14 −0.69 1.0 * 6 5
3A,F Homer1-b/c integrated intensity Homer1-b/c positive synapses (UMAP6 vs UMAP1) Normal Permutation t test 5000 0.003 −0.56 −0.67 −0.43 −5.08 −7.32 −3.58 1.0 * 6 5
b 4A Excitatory synapse number Untreated vs TTX Normal Permutation t test 5000 0.626 2.99 −7.40 15.23 0.29 −1.10 1.59 0.1 6 6
Inhibitory synapse number Untreated vs TTX Normal Permutation t test 5000 0.592 3.62 −7.56 16.24 0.33 −1.03 1.69 0.1 6 6
4B Excitatory:inhibitory synapse ratio Untreated vs TTX Normal Permutation t test 5000 0.592 −0.86 −4.03 1.78 −0.32 −1.64 1.13 0.1 6 6
c 4C vGlut1 integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.006 0.17 0.10 0.24 2.82 1.17 5.03 1.0 * 6 5
vGAT integrated intensity Untreated vs TTX (inhibitory synapses) Normal Permutation t test 5000 0.001 0.60 0.44 0.75 4.31 2.84 6.50 1.0 * 6 5
Synapsin1 integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.110 0.10 −0.02 0.18 1.11 −0.52 4.00 0.4 6 5
Synapsin1 integrated intensity Untreated vs TTX (inhibitory synapses) Normal Permutation t test 5000 0.004 0.35 0.23 0.47 3.11 1.53 4.95 1.0 * 6 5
Bassoon integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.129 0.18 −0.04 0.38 1.02 −0.66 2.49 0.3 6 5
Bassoon integrated intensity Untreated vs TTX (inhibitory synapses) Normal Permutation t test 5000 0.360 0.11 −0.12 0.32 0.57 −0.98 2.07 0.1 6 5
SHANK3 integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.008 0.35 0.12 0.46 2.45 0.60 7.29 0.9 * 6 5
Homer1-b/c integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.037 0.15 0.02 0.26 1.54 −0.02 3.44 0.6 * 6 5
PSD-95 integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.017 0.15 0.06 0.24 1.93 0.71 3.31 0.8 * 6 5
Gephyrin integrated intensity Untreated vs TTX (inhibitory synapses) Normal Permutation t test 5000 0.610 0.07 −0.14 0.35 0.31 −1.24 1.72 0.1 6 5
NR2B integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.089 0.12 −0.02 0.22 1.18 −0.48 3.26 0.4 6 5
Cortactin integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.549 −0.09 −0.33 0.18 −0.39 −2.45 1.07 0.1 6 5
Cortactin integrated intensity Untreated vs TTX (inhibitory synapses) Normal Permutation t test 5000 0.589 −0.09 −0.41 0.24 −0.31 −1.87 1.14 0.1 6 5
Actin integrated intensity Untreated vs TTX (excitatory synapses) Normal Permutation t test 5000 0.475 −0.07 −0.26 0.04 −0.53 −1.80 1.06 0.1 6 5
Actin integrated intensity Untreated vs TTX (inhibitory synapses) Normal Permutation t test 5000 0.794 0.04 −0.22 0.38 0.17 −1.48 2.06 0.1 6 5
d 5C Synapsin1 UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.202 0.13 −0.05 0.30 0.84 −0.72 2.49 0.2 5
Synapsin1 UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.479 −0.03 −0.12 0.03 −0.47 −1.83 1.09 0.1 6 5
Synapsin1 UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.569 0.03 −0.10 0.09 0.36 −1.23 3.70 0.1 6 5
Synapsin1 UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.237 −0.06 −0.17 0.03 −0.82 −2.54 1.03 0.2 6 5
Synapsin1 UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.099 −0.09 −0.18 0.01 −1.09 −2.63 0.48 0.4 6 5
Synapsin1 UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.005 0.37 0.24 0.49 3.09 1.64 4.75 1.0 * 6 5
Gephyrin UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.464 0.02 −0.03 0.07 0.47 −0.92 1.96 0.1 6 5
Gephyrin UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.261 −0.03 −0.08 0.02 −0.68 −2.13 0.68 0.2 6 5
Gephyrin UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.345 −0.03 −0.08 0.02 −0.61 −1.96 0.76 0.1 6 5
Gephyrin UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.304 0.02 −0.02 0.06 0.69 −1.58 2.43 0.2 6 5
Gephyrin UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.647 0.02 −0.05 0.08 0.30 −1.82 2.69 0.1 6 5
Gephyrin UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.733 0.03 −0.12 0.25 0.20 −1.41 1.56 0.1 6 5
Homer1-b/c UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.221 0.09 −0.05 0.23 0.81 −0.89 2.60 0.2 6 5
Homer1-b/c UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.452 0.00 0.00 0.00 −0.81 −1.64 −0.40 0.2 6 5
Homer1-b/c UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.438 0.05 −0.07 0.16 0.49 −1.17 2.16 0.1 6 5
Homer1-b/c UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.660 0.01 −0.04 0.08 0.29 −1.70 2.07 0.1 6 5
Homer1-b/c UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.474 0.02 −0.05 0.08 0.41 −1.23 2.01 0.1 6 5
Homer1-b/c UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.031 0.19 0.07 0.29 1.80 0.48 4.20 0.8 * 6 5
NR2B UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.144 0.10 −0.03 0.22 0.97 −0.48 2.77 0.3 6 5
NR2B UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.827 −0.02 −0.14 0.11 −0.15 −1.71 1.68 0.1 6 5
NR2B UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.312 0.07 −0.04 0.17 0.63 −0.95 2.41 0.2 6 5
NR2B UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.661 0.05 −0.18 0.28 0.26 −1.50 2.35 0.1 6 5
NR2B UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.744 −0.03 −0.20 0.12 −0.20 −1.80 1.34 0.1 6 5
NR2B UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.533 0.14 −0.33 0.54 0.37 −1.08 1.97 0.1 6 5
PSD-95 UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.313 0.06 −0.04 0.17 0.65 −0.85 2.04 0.2 6 5
PSD-95 UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.053 0.06 0.01 0.11 1.43 −0.33 3.18 0.6 6 5
PSD-95 UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.778 0.02 −0.10 0.15 0.17 −1.28 1.56 0.1 6 5
PSD-95 UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.882 0.00 0.00 0.00 −0.12 −1.47 1.58 0.1 6 5
PSD-95 UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.184 0.00 0.00 0.01 0.86 0.40 1.50 0.2 6 5
PSD-95 UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.729 −0.01 −0.08 0.07 −0.21 −1.69 1.26 0.1 6 5
SHANK3 UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.018 0.23 0.11 0.35 2.07 0.81 4.11 0.9 * 6 5
SHANK3 UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.011 0.10 0.04 0.15 1.94 0.55 3.24 0.8 * 6 5
SHANK3 UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.019 0.20 0.08 0.30 1.85 0.55 4.35 0.8 * 6 5
SHANK3 UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.186 0.06 −0.03 0.14 0.85 −0.62 2.44 0.2 6 5
SHANK3 UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.285 0.06 −0.04 0.16 0.67 −0.80 2.17 0.2 6 5
SHANK3 UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.360 0.07 −0.06 0.22 0.58 −0.84 2.15 0.1 6 5
Actin UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.345 −0.10 −0.28 0.06 −0.60 −1.95 1.11 0.1 6 5
Actin UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.927 0.00 −0.05 0.06 −0.06 −1.54 1.59 0.1 6 5
Actin UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.407 −0.06 −0.18 0.06 −0.56 −2.01 1.13 0.1 6 5
Actin UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.721 −0.01 −0.07 0.05 −0.21 −1.70 1.26 0.1 6 5
Actin UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.923 −0.01 −0.18 0.07 −0.11 −1.72 2.14 0.1 6 5
Actin UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.876 0.01 −0.14 0.19 0.10 −1.31 1.65 0.1 6 5
Bassoon UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.250 0.08 −0.04 0.21 0.74 −0.90 1.94 0.2 6 5
Bassoon UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.593 −0.02 −0.11 0.06 −0.33 −1.97 1.56 0.1 6 5
Bassoon UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.064 0.09 0.02 0.22 1.16 −0.33 2.12 0.4 6 5
Bassoon UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.877 0.01 −0.09 0.13 0.09 −2.47 3.45 0.1 6 5
Bassoon UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.569 0.02 −0.05 0.08 0.33 −1.26 2.33 0.1 6 5
Bassoon UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.281 0.07 −0.06 0.19 0.64 −0.83 2.20 0.2 6 5
Cortactin UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.201 −0.09 −0.24 0.01 −0.84 −2.03 0.63 0.2 6 5
Cortactin UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.573 −0.02 −0.11 0.05 −0.34 −1.81 1.07 0.1 6 5
Cortactin UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.390 0.00 0.00 0.00 0.55 −0.98 2.37 0.1 6 5
Cortactin UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.867 0.00 0.00 0.00 0.11 −1.21 1.57 0.1 6 5
Cortactin UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.781 −0.02 −0.12 0.11 −0.16 −2.09 1.78 0.1 6 5
Cortactin UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.585 −0.06 −0.24 0.14 −0.33 −1.87 1.10 0.1 6 5
vGlut1 UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.044 0.19 0.01 0.30 1.49 −0.25 3.75 0.6 * 6 5
vGlut1 UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.252 0.03 −0.02 0.08 0.78 −0.83 2.69 0.2 6 5
vGlut1 UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.045 0.08 0.01 0.16 1.38 −0.39 3.00 0.5 * 6 5
vGlut1 UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.231 0.03 −0.02 0.07 0.77 −0.83 2.44 0.2 6 5
vGlut1 UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.722 0.01 −0.05 0.09 0.21 −2.28 3.33 0.1 6 5
vGlut1 UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.358 −0.01 −0.02 0.01 −0.57 −2.24 1.05 0.1 6 5
vGAT UMAP1 Untreated vs TTX Normal Permutation t test 5000 0.386 0.00 −0.01 0.00 −0.60 −1.69 1.01 0.1 6 5
vGAT UMAP2 Untreated vs TTX Normal Permutation t test 5000 0.883 0.00 0.00 0.00 0.08 −1.41 1.55 0.1 6 5
vGAT UMAP3 Untreated vs TTX Normal Permutation t test 5000 0.101 −0.01 −0.02 0.00 −1.03 −1.98 0.55 0.3 6 5
vGAT UMAP4 Untreated vs TTX Normal Permutation t test 5000 0.861 0.00 0.00 0.01 0.06 −1.31 1.57 0.1 6 5
vGAT UMAP5 Untreated vs TTX Normal Permutation t test 5000 0.101 −0.01 −0.01 0.00 −1.13 −2.16 −0.49 0.4 6 5
vGAT UMAP6 Untreated vs TTX Normal Permutation t test 5000 0.002 0.86 0.61 1.08 3.99 2.55 6.30 1.0 * 6 5
e 5C UMAP1 synapse number Untreated vs TTX Normal Permutation t test 5000 0.117 0.11 0.01 0.22 1.09 −0.56 2.56 0.4 6 5
UMAP2 synapse number Untreated vs TTX Normal Permutation t test 5000 0.042 −0.27 −0.47 −0.04 −1.52 −3.59 0.15 0.6 * 6 5
UMAP3 synapse number Untreated vs TTX Normal Permutation t test 5000 0.092 0.23 0.07 0.53 1.17 0.10 2.19 0.4 6 5
UMAP4 synapse number Untreated vs TTX Normal Permutation t test 5000 0.091 −0.14 −0.28 0.03 −1.13 −3.51 0.56 0.4 6 5
UMAP5 synapse number Untreated vs TTX Normal Permutation t test 5000 0.002 −0.42 −0.60 −0.30 −3.12 −4.32 −1.96 1.0 * 6 5
UMAP6 synapse number Untreated vs TTX Normal Permutation t test 5000 0.428 0.12 −0.11 0.42 0.51 −0.84 1.85 0.1 6 5

Two-tailed permutation t tests, mean confidence intervals, and effect size were calculated the from 5000 reshuffles of the untreated (n = 6) and TTX-treated (n = 5) samples. The p values represent the likelihood of observing the reported effect size, if the null hypothesis of zero difference is true. Power analysis represents the probability of observing effect size if the null hypothesis of zero difference is true.