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. 2021 Jan 13;8(1):ENEURO.0011-20.2020. doi: 10.1523/ENEURO.0011-20.2020

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Copyright © 2021 Kadam et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 1. — Expression of Psrx-97::mCherry in ASH and PHB neuron. A, Cartoon image showing the location of the amphid and phasmid neurons in C. elegans. B, Expression of the Psrx-97::mCherry transgenic construct in the whole animal. C, srx-97 promoter (600 bp) expression in a single pair of amphid and phasmid neurons. D, The expression of the srx-97 promoter (2 kb) in a pair of amphid neurons and (E) phasmid neurons in C. elegans. F, Expression of Psrb-6::GFP and Posm-10::GFP in their respective neurons (indicated on the figure) and their co-localization with Psrx-97::mCherry in the amphid ASH neurons. G, Expression of Posm-10::GFP in its respective neurons (indicated on the figure) and their co-localization with Psrx-97::mCherry in the phasmid PHB neuron. The lower panel indicates a DIC image indicating the position of the PHA and PHB neurons. H, Expression of SRX-97::mCherry in the cell bodies (dotted circles) and SRX-97 localization to the cilium tip of the ASH neurons (between the dotted circles in the figure to the left).