Figure 2.

The SRX-97 transmembrane domain and CRISPR/Cas9 generated mutation of srx-97. A, Amino acid sequence showing the predicted seven transmembrane domain of SRX-97. B, Exonic structure of the srx-97 gene with the red line showing the CRISPR/Cas9 deletion obtained. The deletion encompasses the gene from the 61st base pair to the 1895th base pair including part of the 3′ UTR of the gene. C, Amplification of the chromosomal region showing the deletion of the srx-97 gene (2730 bp) using CRISPR/Cas9 compared with control WT (4566 bp) gene. A 1-kb DNA ladder was used in the line marked Marker (M). Extended Data Figure 2-1 supports this figure.