Figure 3.

Behavior of srx-97 mutant animals toward water-soluble and volatile chemicals. A, Graph showing the delay in avoidance response toward a dry spot of 2 m glycerol in WT, srx-97, and odr-3 mutant animals. The number of animals assayed for each genotype is indicated at the base of each plot for panels A–D. B, Graph showing the delay in avoidance toward a dry spot of 0.1% SDS in WT, srx-97, and odr-3 mutant animals. C, Graph showing the delay in avoidance toward a dry spot of 10 mm CuSO₄ in WT, srx-97, and odr-3 mutant animals. D, Graph showing the percentage of avoidance on nose touch stimuli of WT, srx-97, and glr-1 mutant animals. The numbers at the base of graphs in A–D indicate the number of animals tested for each genotype. E, Graph indicating the negative chemotaxis indices of WT, srx-97, and odr-3 mutant animals toward the repellent octanol. The assay was done in triplicates over multiple days for all chmotaxis assays. Each dot indicates an assay done in triplicate for all graphs from E–H. F, Chemotaxis indices toward high concentrations (10⁻¹) of DA and IAA. G, Chemotaxis indices toward multiple concentrations of benzaldehyde. H, Chemotaxis indices toward high concentrations of benzaldehyde in WT, srx-97, and rescue strains of srx-97. This rescue experiments used SRX-97 under its own promoter and under the osm-10 promoter. Animals that did not show expression of the arrays (NA, no array) were used as controls in these experiments. The error bars represent SEM, and statistical significance is represented as “ns” for not significant, *p < 0.05, **p < 0.01, ***p < 0.001. The numbers at the base of each graph from E–H indicates the total number of times the experiment was performed with 50–150 animals used in each trial. Extended Data Figure 3-1 supports this figure.