Figure 4.

Ablation of ASH neurons shows defects toward chemosensation to benzaldehyde. A, Illustration of the design of the plates used for analyzing the chemotaxis frequency of C. elegans along with the formula used for this calculation. Each sector (a–d) is 1 cm in width. B, Graph of chemotaxis frequencies of WT, srx-97, the srx-97 rescue line and a control odr-3 mutant line to a high concentration of benzaldehyde. The assay was performed in triplicate over multiple days with each dot indicating an assay done in triplicate. The numbers at the base of each plot indicate the number of times the experiment was performed with each genotype. C, Graph plotting the delay in response of animals toward a high benzaldehyde concentration. The animals used in this experiment have undergone mock ablation or ASH ablation in WT or srx-97 mutant backgrounds. Each dot indicates a response from a single animal. Approximately 25 mock ablated animals and ASH ablated animals in WT and srx-97 mutant background were analyzed for this experiment over multiple days. The error bars represent SEM, and statistical significance is represented as “ns” for not significant; *p < 0.05, **p < 0.01, ***p < 0.001.