Table 1.
Primers used in this study
| Primer number | FP/RP | Primer sequence | Gene name | Vector backbone |
|---|---|---|---|---|
| NK181 NK196 |
FP RP |
atcagcatgcatcttgaaaacctcaatcgaaccag ctctctcccgggggacatatcttgaaagtttggaatggag |
Psrx-97 | pPD49.26_mCherry |
| NK214 NK196 |
FP RP |
ctctctcgcatgcggtaagttttgcagtctaggcag ctctctcccgggggacatatcttgaaagtttggaatggag |
Psrx-97 | pPD49.26_mCherry |
| NK259 NK260 |
FP RP |
ctctctgcatgcggaaccgtatttttgtgcaatagtcg ctctctcccggggcaagatgaaatttccaaaaaagtttattgatatgg |
Posm-10 | pPD95.75 |
| NK262 NK263 |
FP RP |
ctctctgcatgcgccaaaactgctgaacttttg ctctctcccgggcttctgtagaaatttcaagactgatcac |
Psrb-6 | pPD95.75 |
| NK197 NK279 |
FP RP |
ctctctcccgggatgtccttatcgaattggacgc ctctctggtaccttcaacatgatcctattcaagtttggtatttttc |
srx-97_UTR | pPD49.26 |
| NK197 NK254 |
FP RP |
ctctctcccgggatgtccttatcgaattggacgc ctctctggtacctcaaaatgtgactgttaaaactgtgactt |
srx-97 gene | pPD49.26_mCherry |
| gRNA_1 | atcaggtctcctcttccccacttatgactattacagttttagagctagaaatagcaag | srx-97 gene | pRB1017 | |
| gRNA_2 | atcaggtctcctcttaaaattataaggcgtaggcagttttagagctagaaatagcaag | srx-97 gene | pRB1017 | |
| Homology amr_1 | FP RP |
atcatctagatcttccggacgtgaatcttctatc atcagcatgcatcttgaaaacctcaatcgaaccag |
srx-97 gene | pPD95.75 |
| Homology amr_2 | FP RP |
atcagggccccattgcacaactgataagatagtgc atcacttaagttcaacatgatcctattcaagtttggt |
srx-97 gene | pPD95.75 |
| NK263 NK264 NK265 |
EFP IFP RP |
ctacagtttagtgcttgccacag tgtcgaaattaaagggtttcgagg gctgtccaacgcaattttcg |
gpc-1 | genotyping |
| NK303 NK304 NK279 |
EFP IFP RP |
gctaggtggagggctgattg gctaggtggagggctgatta atgggcggtggaagttcg |
osm-9 | genotyping |
| NK207 NK209 |
FP RP |
ggatagaagattcacgtccggaag tccatgtggggtttgctctg |
srx-97 | genotyping |
| NK210 NK179 |
FP RP |
cttccaacatgaaaagcactatcttatcag ttcaacatgatcctattcaagtttggt |
srx-97 | genotyping |
| PRS394 PRS395 PRS396 |
FP ERP IRP |
gcggttcggatacgaaaatacttg gacggagaagtgtatccgttatatc ccatgcgtccgtccctaatcc |
tax-4 | genotyping |
| PRS442 PRS443 PRS444 |
FP ERP IRP |
cactggcgacgattgtcagatc gttatttccagtagatgtggccacg gtagccaaaatgagttgatctg |
tax-2 | genotyping |
| AB37 AB38 AB39 |
FP-WT FP-glr-1 RP |
acctttcggctccgacttg acctttcggctccgactta attgaaatgaccataccacc |
glr-1 | genotyping |
FP, forward primer; RP, reverse primer; FP-WT, external forward primer for WT glr-1 sequence; FP-glr-1, external forward primer for glr-1 sequence in the glr-1 point mutation line.