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. 2021 Jan 28;11:596263. doi: 10.3389/fphys.2020.596263

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Copyright © 2021 Bloomfield, Chen and Cimini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tetraploid DLD-1 clones have distinct mitotic spindle geometries. (A) Examples of mitotic spindles from parental DLD-1 cells and cells from 4N DLD-1 clones S1, S2, L1, and L2 (top-to-bottom) in metaphase. Scale bar, 5 μm. Measurements of mitotic spindle width (B), height (C), length (D), and volume (E) reported as mean ± SEM with individual data points from three independent experiments in which a total of 30–40 cells (n = 35, 30, 40, 30, and 35, respectively). **p < 0.01, ****p < 0.0001, when compared to the parental DLD-1 cells by Student's t-test.