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. 2021 Jan 28;11:596263. doi: 10.3389/fphys.2020.596263

Figure 7.

Figure 7

Blebbistatin treatment modifies spindle dimensions and reduces metaphase duration in S1 cells. (A–D) Measurements of mitotic spindle width (A), height (B), length (C), and volume (D) in untreated and blebbistatin-treated S1 cells (n = 29 and 36, respectively), reported as mean ± SEM with individual data points from three independent experiments. ***p < 0.001, ****p < 0.0001, when compared to untreated cells by Student's t-test. (E) Model-predicted metaphase duration using the median spindle dimensions in panels (A–C). Cell size, spindle pole size, and spindle microtubule abundance were assumed to be the same between the control and treated groups. (F–H) Quantification of (F) mitotic duration in untreated and blebbistatin-treated S1 cells (n = 167 and 193, respectively), (G) prometaphase duration in untreated and blebbistatin-treated S1 cells (n = 101 and 92, respectively), and (H) metaphase duration in untreated and blebbistatin-treated S1 cells (n = 101 and 94, respectively). ***p < 0.001, ****p < 0.0001, when compared to untreated cells by Student's t-test.