Cell cycle entry and distribution of WT and arid4bΔ mESCs.
FACS histogram of a. wild type (CJ9) b. arid4bΔ endoderm directed cells determined by Ki-67-eFluor660 staining. c. Bar graph representation of Ki-67 positive WT and arid4bΔ endoderm directed cells. (Paired t-test: P < 0.05) d. Cell cycle phase distribution of Ki67-positive WT and arid4bΔ endoderm directed cells.
Error bars indicates standard error. (Unpaired t-test: G1: P < 0.01, S: P < 0.01, G2: not significant)
FlowJo cell cycle analysis of e. WT and f. arid4bΔ endoderm directed cells stained with Ki67-FITC and Black line represents the experimental analysis, while pink trace is cell cycle fit determined by the FlowJo software cell cycle suite. Green, yellow and blue shaded areas indicate G1, S and G2 phases, respectively.