Fig 1. TRIM22 depletion increases HSV-1 viral yields and gene expression.

HFFs were transfected with siRNA pools specific for TRIM22 or non-targeting siRNAs. Transfected HFFs were treated with PBS or hIFNα-2a at 1000U/ml for 24 h. These HFFs were then infected with HSV-1 ICP0-null (7134) or the rescued virus (7134R) at an MOI of 5 for 24 h. (A) Virus yields were measured at 24 hpi using plaque assays on U2OS cells (n = 3). Error bars represent standard errors of the means (P<0.05*, P<0.005**, P<0.0002***,P<0.0001**** Two-way ANOVA, multiple comparisons with Tukey’s corrections). (B) TRIM22 transcript levels were measured by qRT-PCR with primers specific for TRIM22 transcripts and 18S rRNA (n = 3). (C) Protein levels were determined by immunoblotting for TRIM22 and GAPDH at time of infection.