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. 2021 Feb 1;17(2):e1009339. doi: 10.1371/journal.pgen.1009339

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© 2021 Syx et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Steady-state biochemical (pro)collagen analysis of (pro)collagens harvested from medium and cell layer from dermal fibroblast cultures. (B) Intracellular folding assay for type I collagen measured by pulse-chase labeling of type I collagen with AHA-Alexa Fluor 488 and staining of the total amount of type I collagen (with and without AHA) with GelCode Blue Stain Reagent. ‘-‘ denotes Alexa Fluor 488 labeled TBS buffer only, Ctrl: purified AHA incorporated type I collagen from culture medium after overnight chase as a loading control. (C) Type I collagen secretion assay. (D) Thermal stability of secreted type I collagen molecules with biochemical analysis. (E) Melting curve analysis of type I collagen measured in the control (black) and patient (pink) by circular dichroism measurements. C: control, P: patient.