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. 2021 Feb 1;17(2):e1009211. doi: 10.1371/journal.ppat.1009211

Fig 6. TgQCR11 is important for Complex III integrity.

Fig 6

(A-D) Western blots of proteins extracted from (A) rTgQCR11-FLAG/TgMPPα-HA, (B) rTgQCR11-FLAG/TgQCR12-TEV-HA, (C) rTgQCR11-FLAG/TgQCR8-TEV-HA and (D) rTgQCR11-FLAG/TgCytC1-HA parasites grown in the absence of ATc or in the presence of ATc for 1–3 days. Samples were prepared in 1% (w/v) DDM, separated by BN-PAGE, and detected with anti-HA antibodies. (E-H) Western blots of proteins extracted from (E) rTgQCR11-FLAG/TgMPPα-HA, (F) rTgQCR11-FLAG/TgQCR12-TEV-HA, (G) rTgQCR11-FLAG/TgQCR8-TEV-HA and (H) rTgQCR11-FLAG/TgCytC1-HA parasites that had been grown in the absence of ATc or in the presence of ATc for 1–3 days. Samples were separated by SDS-PAGE, and probed with anti-HA, anti-FLAG and anti-TgTom40 (loading control) antibodies. Western blots shown are representative of at least two independent experiments, with matched BN-PAGE and SDS-PAGE samples prepared from the same experiment. Quantifications of protein abundances are depicted in S13 Fig.