Fig 1. Design and fabrication of nanowell-arrayed microscope slides for the compartmentalization and visualization of TB bioaerosols.

(A) Photograph and (B) schematic of a nanowell-arrayed microscope slide. (C) 3D scan of a 207.840 x 277.029 μm section of the slide. Each device (25 mm x 75 mm) consists of two rows of eight round microwells machined from cast acrylic. The microwells are 6 mm in diameter and 2 mm deep. The nanowell film, which is bonded to the superstructure with UV-curing adhesive, is made from embossed COC film. The nanowells have side-wall angles of 35° and are 50 μm deep. The distance through the bottom of each well to the back of the film is ~170 μm, equivalent to a number 1.5 coverslip.