Table 2.

Pharmacokinetic effects combining N‐GCS and Fc mutations

Construct	Signal sequence	Catalytic domain	Nuclease domain	Fc domain	Half‐life, hours	AUC, mOD/min hour
8			S766N	M883Y S885T T887E	45	1,912
770					37	3,382
9	V29N		S766N	H1064K N1065F	65	6,047
10	C25N K27T		S766N	H1064K N1065F	55	7,735
11	V29N			H1064K N1065F	57	14,506
12	V29N			H1064K N1065F	63	13,812
13	V29N		E592N	M883Y S885T T887E	70	14,978
14				M883Y S885T T887E	95	20,360
15	V29N		E592N	H1064K N1065F	99	22,690
7		I256T			66	26,596
17	V29N	I256T	P534N V536T	M883Y S885T T887E	120	33,751

The color shades correspond to the domain coloring of the ENPP1 cartoon presented in Figure 1a, i.e., the listed mutations in the table are colored according to their domain location in the cartoon in figure 1a.

AUC, area under the curve; mOD, measured as the optical density; N‐GCS, N‐glycan consensus sequences.